Pepsi Corporation History and Major Problems Essay Example

Pepsi Corporation History and Major Problems Essay I. PEPSI CORPORATION HISTORY The Pepsi-Cola story began in a drugstore in New Bern, North Carolina, USA in 1896. Caleb Davis Bradham, a pharmacist, owned a drugstore named Bradham Drug Company in downtown New Bern. Like many of the drug stores at the time, Bradham’s store also housed a soda fountain. Here, Bradham offered his own soda concoctions which attracted most of the small-town folks. On August 28, 1898, Bradham invented a new recipe, blending kola nut extract, vanilla and â€Å"rare oils. This soda drink, initially known as Brad’s Drink, gathered so much popularity not only for its delicious and refreshing taste but people believed it helped them by aiding digestion and boosting their energy. Bradham likened the effect to digestion to the enzyme pepsin, even though it was not used as an ingredient. By combining the terms pepsin and cola, Bradham renamed his recipe Pepsi-Cola. Pepsi’s early growth was less significant than that of Cokes, and its real stre ngth as a competitor to Coke began after became CEO in 1950, a time when Pepsi was nearly bankrupt. Bradham was expected to liquidate the Pepsi-Cola Co. Instead, he made it his goal to beat Coke (, 2003). Pepsi spread to Canada in 1906, and the year after that, it was registered in Mexico. By 1908, Pepsi had upgraded their transportation delivery services from horse-drawn carts to automobiles. Pepsi seemed to be becoming successful in such a short time. But there is still a problem. By the time Pepsi Cola came out, Coke had already made its mark among the public and the newcomer Pepsi found it hard to find a consumer base. In the 1920’s, the company released the ad Drink Pepsi. It will satisfy you. However, despite industrious efforts, the Pepsi Company still fell into bankruptcy due to the fall of the sugar market. After seventeen years of success, Caleb Bradham lost Pepsi Cola. He had gambled on the fluctuations of sugar prices during W. W. I, believing that sugar prices would continue to rise but they fell instead leaving Caleb Bradham with an overpriced sugar inventory. Pepsi Cola went bankrupt in 1923. We will write a custom essay sample on Pepsi Corporation History and Major Problems specifically for you for only $16.38 $13.9/page Order now We will write a custom essay sample on Pepsi Corporation History and Major Problems specifically for you FOR ONLY $16.38 $13.9/page Hire Writer We will write a custom essay sample on Pepsi Corporation History and Major Problems specifically for you FOR ONLY $16.38 $13.9/page Hire Writer It suffered several years of losses before it was sold to Loft Inc. , a giant candy company. Loft was what Pepsi was waiting for. The company began to regain its former success. 1936 saw the formation of Pepsi Limited of London, and in 1938, the Pepsi logo was trademarked in the Soviet Union. The company sold the drink in 12 oz. bottles and launched the advertising campaign of Twice as Much for a Nickel. In 1993, Pepsi-Cola found itself in a crisis situation when a man in Tacoma, Washington claimed he had found a syringe inside a can of Diet Pepsi. Soon after the story hit the news, claims surfaced all over the country. People claimed to find objects from bullets to crack cocaine vials. Pepsi-Cola knew that the foreign objects had been inserted by people outside the company who had tampered with the product. Pepsi-Cola decided to use a defensive strategy, claiming its innocence in the matter. Proving the company’s innocence would be pivotal in protecting further damage to Pepsi-Cola’s brand name. Pepsi employed a variety of strategies to deal with the problem. PepsiCos India operations were met with substantial resistance in 2003 and again in 2006, when an environmental organization in New Delhi made the claim that, based on its research, it believed that the levels of pesticides in PepsiCo (along with those from rival Coca-Cola Company), exceeded a set of proposed safety standards on soft drink ingredients that had been developed by the Bureau of Indian Standards. PepsiCos usage of water was the subject of controversy in India in the early and mid 2000s in part because of the companys alleged impact on water usage in a country where water shortages are a perennial issue. In this setting, PepsiCo was perceived by India-based environmental organizations as a company that diverted water to manufacture a discretionary product, making it a target for critics at the time. Pepsi-Cola came to the Philippines through John Clarkin, a native of Minnesota, USA, who came with the American forces at the close of World War II. On October 16, 1946, Clarkin acquired a franchise to bottle Pepsi in the Philippines. He consequently founded the Pepsi-Cola Bottling Company of the Philippine Islands Ltd. and became its president and general manager. As of January 2012, 22 of PepsiCos product lines generated retail sales of more than $1 billion each,and the companys products were distributed across more than 200 countries, resulting in annual net revenues of $43. 3 billion. Based on net revenue, PepsiCo is the second largest food beverage business in the world. Within North America, PepsiCo is ranked (by net revenue) as the largest food and beverage business. II. MAJOR PROBLEMS 1. COMPETITORS â€Å"Pepsi’s early growth was less significant than that of Cokes, and its real strength as a competitor to Coke began after someone became CEO in 1950, a time when Pepsi was nearly bankrupt. Bradham was expected to liquidate the Pepsi-Cola Co. Instead, he made it his goal to beat Coke (, 2003). Pepsi spread to Canada in 1906, and the year after that, it was registered in Mexico. By 1908, Pepsi had upgraded their transportation delivery services from horse-drawn carts to automobiles. Pepsi seemed to be becoming successful in such a short time. But there is still a problem. By the time Pepsi Cola came out, Coke had already made its mark among the public and the newcomer Pepsi found it hard to find a consumer base. 2. FINANCIAL PROBLEM/BANKRUPTCY â€Å"In the 1920’s, the company released the ad Drink Pepsi. It will satisfy you. However, despite industrious efforts, the Pepsi Company still fell into bankruptcy due to the fall of the sugar market. After seventeen years of success, Caleb Bradham lost Pepsi Cola. He had gambled on the fluctuations of sugar prices during W. W. I, believing that sugar prices would continue to rise but they fell instead leaving Caleb Bradh am with an overpriced sugar inventory. Pepsi Cola went bankrupt in 1923. 3. PUBLIC RELATIONS CRISIS â€Å"In 1993, Pepsi-Cola found itself in a crisis situation when a man in Tacoma, Washington claimed he had found a syringe inside a can of Diet Pepsi. Soon after the story hit the news, claims surfaced all over the country. People claimed to find objects from bullets to crack cocaine vials. Pepsi-Cola knew that the foreign objects had been inserted by people outside the company who had tampered with the product. Pepsi-Cola decided to use a defensive strategy, claiming its innocence in the matter. Proving the company’s innocence would be pivotal in protecting further damage to Pepsi-Cola’s brand name. Pepsi employed a variety of strategies to deal with the problem. † 4. SAFETY STANDARDS â€Å"PepsiCos India operations were met with substantial resistance in 2003 and again in 2006, when an environmental organization in New Delhi made the claim that, based on its research, it believed that the levels of pesticides in PepsiCo (along with those from rival Coca-Cola Company), exceeded a set of proposed safety standards on soft drink ingredients that had been developed by the Bureau of Indian Standards. 5. WATER USAGE â€Å"PepsiCos usage of water was the subject of controversy in India in the early and mid 2000s in part because of the companys alleged impact on water usage in a country where water shortages are a perennial issue. In this setting, PepsiCo was perceived by India-based environmental organizations as a company that diverted water to manufacture a discretionary product, making it a target for critics at the time. †

Do these cliché career tips actually work

Do these clichà © career tips actually work Does this sound familiar? You’re trying to figure out your future and you’re in the midst of some serious career planning, when all of a sudden you’re inundated on all sides by a hurricane of advice on what to do, what not to do, and how to move forward. The truth is, when it comes to career planning most people think they have all the right answers when they’re dishing out clichà ©d and uninformed advice to others, and if you’re the unwitting recipient of these tired tips from everyone in your life you’re likely feeling overwhelmed and unsure of where to turn.Don’t bother doing a blind search on the Internet for career tip guidance- you’ll only encounter the same sort of tired advice that contradicts itself and takes all sides and forms depending on where you look. So, with all of this â€Å"career noise† at every turn, how do you know what to believe and follow?You should always do what you love.This is a nice thought, and it would be an interesting world if everyone got to do exactly what they want to do or love. But the truth is, reality sometimes holds a different view of how our careers should play out. While it can be a good idea to pursue a dream, it can be foolhardy to pursue it forever- after all, a noble pursuit like following your dream won’t pay your bills. Besides, it’s possible to find happiness and fulfillment while pursuing a backup plan. Use logic and reality as your guide when deciding between pursuing your dream and choosing an acceptable alternative.Don’t leave your current job until you have a new one lined up.This job advice tidbit is as old as it gets- but does that necessarily mean it’s sound advice? This tip gets particularly murky if your job environment is especially toxic or unhealthy. Sure, it makes sense to have a steady paycheck and job history while job hunting and until something new comes along, but life is short and if you’re re ally in a difficult or miserable spot, then it may be worthwhile to see if you can survive if you left your job. Besides, it’s easier to job hunt when you have more time to focus on it. Once again, fully weigh your situation, along with the pros and cons, and decide wisely.Don’t leave a job before you’ve been there for at least a year.Everyone has heard this one before, but is it actually sound advice? Employers like to work with people who stick around since it’s so expensive to recruit and train them.†However, they also suggest that reality might not be so black and white: â€Å"While you want to avoid having  lots  of short stays in your job history, most people will understand if there was a particular situation that wasn’t great. If it’s your boss or the day-to-day nature of your work that’s  not what you expected, keep in mind that there are sometimes options to change positions without leaving the organization. Sta rt by looking for new responsibilities and discussing a  transfer to another team. If, after that, you’re still itching to get out, update your resume and re-start your job hunt.†If you change your field you’ll have to start at the bottom.Are you finding yourself in a position where you’re thinking of a complete career overhaul? If so, then you’ve undoubtedly encountered this advice, which came from the notion that you always have to â€Å"pay your dues† when starting out in an industry and work your way up from the bottom. But whoever originally came up with this one obviously never thought about the value of transferable work experience and skills. According to The Muse, â€Å"if you’ve already put time into your current career, you probably have more skills that transfer from field to field than you recognize. Not just that, but your years of work have also taught you how to network, which can play a role in getting off the groun d floor.† Bottom line: recognize your value and transferable skill set when considering what positions in other fields make sense for you, and don’t let this tired old clichà © deter you from pursuing a new career in a new field.There you have it- a fresh look on some old career tip clichà ©s. Use the information provided here to help declutter your brain when it gets too full of competing advice and you’ll be on your way to make smart career decisions in no time.

Sociology .. just paraphrase Essay Example | Topics and Well Written Essays - 1500 words

Sociology .. just paraphrase - Essay Example f)Master status. This refers to a particular status in society that automatically ranks above all others, placing the individual with these characteristics within the top element of his or her society. g)Status symbols. Status symbols are specific material clues that refer to an individual’s position in society such as a doctor with a stethoscope or an athlete with a basketball. h)Roles. Roles are specific patterns of behavior that are expected of people who fall within a specific social status position. K) Major institutions. A major institution is any institution as defined above that are significantly large and well organized such as a school system or the national government. n)Mechanical solidarity. This refers to a situation in which things happen essentially in the same way they have always happened bringing about social cohesion based on shared experience and knowledge skills. o)Organic solidarity. Organic solidarity suggests there is a sort of collective consciousness which exists due to society’s inescapable mutual interdependence which exists in proportion to that society’s complexity in its division of labor. The theory that Goffman presents suggests that the forces that shape who we become, who we are and how we will act in various situations is significantly affected by the people with whom we associate. According to this theory, each individual has a tendency to present ourselves as what we want others to think of us as if we are permanently on stage. In keeping with the metaphor, he suggests we spend a great deal of time rehearsing our performances in the backstage action using the life script we are given by our society prior to our ‘live’ performance. This performance, again like that seen on a stage, also requires the participation of other characters/cast/individuals if it is to be successfully presented to

Patient protection and affordable care act(H.R. 3590) Research Paper

Patient protection and affordable care act(H.R. 3590) - Research Paper Example In this paper, facts about the components of the new law will be discussed, with its corresponding advantages and disadvantages. H.R. 3590, also known as â€Å"The Patient Protection and Affordable Healthcare Act,† has been in the public spotlight for months before and after the president ordered its issuance. According to the Democratic Public Policy (n.d.), the current health care reform will â€Å"ensure that all Americans have access to quality, affordable health care,† and allows modification within the health care service to fit whatever individual medical needs. The role of the health insurance holders is also emphasized, making them more in-control of their insurance companies, rather than insurance companies controlling them. The Kaiser Family Foundation (2010) argued that the change in the health care system â€Å"focuses on the provision of to expand coverage, control health care costs, and improve health care delivery system.† Its implementation would rely to the overall population with health insurance coverage; therefore, there is a need to make health insurance mandatory for every citizen. If a citizen fails to have such insurance a penalty of $695 tax should be paid, but varies in accordance to his or her marital status. In line with the widened coverage for health insurance, the new policy provides a longer coverage for young adults under their parent’s premiums. The Los Alamos National Laboratory (n.d.) contends that the employees or unemployed young adults who lost their insurance coverage on their 23rd birthday will be allowed to re-enroll under their parent’s insurance, regardless if they are married or not. Furthermore, the previous policy of insurance companies of disqu alifying children who have pre existing medical condition is already illegal. A 1% budget for the Abortion policy will be provided, as long as the pregnancy is due to rape or incest, or the pregnancy itself causes danger

Final Reflective Assignment Example | Topics and Well Written Essays - 2500 words

Final Reflective - Assignment Example Cascio (2006) defined human resource policies as the process of organizing the resource planning, performance management, employee relations and supporting administrative roles. This report will focus on identifying the process and importance of human resource policies within in the organizational framework. The researcher will also try to evaluate the effectiveness of these policies within the organizational context. The process of developing human resource policies depend on the specific needs of a firm and its employees. Most of the firm try to develop their human resource polices by relating them to their organizational objectives which provides an explicit direction to the workforce (Adeniji & Osibanjo, 2012). The influence of the policies can either be negative or positive based on the effectiveness of implementing them. For instance, Microsoft Corp.’s HR policy of stack ranking is criticized as one of the worst policies (Burstein, 2013). The policy was focused on ranking the employees based on their performance which took an ugly form and emerged in the form of organizational politics and decreasing productivity (Burstein, 2013). Focusing on the process of policy making, the basic aspects of policy framing and drafting will be referred in the report. The first step for policy making is to identify the need. This step involves all the HR personnel of the company. The line managers will collect the data on the progress, performance and productivity of the employees and forward it to the senior managers (Cascio, 2006). The senior managers analyze the data and identify the gaps in the work culture and work structure. Based on this, a basic framework for the policy is developed and further forwarded to the higher management of the company. This is where the policy framework is compared and aligned with the strategic decision making process (McKenzie & Wharf, 2010). The second step is to assess the scope of the

PKR-eIF2α Signaling Mediated Spatial Memory Impairment

PKR-eIF2ÃŽ ± Signaling Mediated Spatial Memory Impairment SUPPLEMENTARY MATERIAL Activated PKR-eIF2ÃŽ ± signaling mediated spatial memory impairment, tau phosphorylation, AÃŽ ² pathology, oxidative stress, selectively synaptic protein loss in mice caused by low levels of Cu Mouse behavior analysis. Morris water maze test: MWM was performed as previously described (PMID:23402899) with minor modifications and the test was performed double blinded according to the standard operation protocol. The MWM consisted of a circular pool (120 cm diameter, wall depth 40 cm) in which all the mice were trained to escape from water by swimming to a hidden platform (2 cm beneath water surface) whose location only be identified by using the visual cures on the inner wall of the pool (Supplementary Fig. 3A). The water and the room temperature were kept at 23 ±1 à ¢Ã¢â‚¬Å¾Ã†â€™. The pool was divided into four quadrants by a computerized tracking software (Huaibei Zhenghua Biologic Apparatus Facilities Limited Company, Huaibei, Anhui, China). The platform was located half-way between the center and the wall in one quadrant and maintained at the same position during all the experiment. The navigation test consisted of 4 training trials per day and 5 consecutive training days. As can be seen in Table 1, mice were released with their heads facing the inner wall of the pool from the four quadrantal locations (N, E, SE, and NW) according the sequence as previous report (Supplementary Fig. 3B) (PMID:17406317), and not allowed to swim and search for the platform more than 60 s, after which they were guided to the platform and allowed to remain on it for 15 s. Each mouse was then returned to its cage for 30 min before its next trial. The latency to reach the hidden platform was recorded. One day or six days after the end of navigation test, mice received a probe test, in which the platform was removed. Mice were released from the NE location and allowed to a 120 s swim to find the previous location of the platform. The swimming path, the time spent in each quadrant, the distance traveled each quadrant, the probe time, the platform crossing number, the total distance traveled, and the average swimming speed was recorded by the computerized tracking software. Y-maze: In order to study the PKR role in exploratory behavior and spatial memory, we performed the Y-maze in the PKR+/+Tg+/- and PKR+/-Tg+/- mice as described previously (PMID: 8986335, 1393562). Response to novelty was tested in a Y-maze, adopting a two-trial procedure in this test. The apparatus was equipped with black materials with three identical arms each 50 cm long, 16 cm wide, and 32 cm high. Visual cues made from colored paper with different symbols and the floor of the maze was covered with soiled animal bedding (Beta wood chips). All the mice was performed with starvation treatment for 24h before Y-maze. In trial 1, one arm was blocked with black Plexiglas and referred to as the â€Å"novel† arm in Trial 2. The remaining two arms were designated as the ‘start’ arm and â€Å"other† arm respectively. Three arms were randomized between mice (but not for the same mouse) to reduce arm bias effects. At the start of testing, a mouse was placed in the start arm and was allowed to explore the start and other arms for 10 min (acquisition trial). At the end of Trial 1, the mouse was returned to its home cage and the bedding inside the maze was mixed to reduce the possibility of using odors as a cue. After an intertrial interval (ITI) of 1 h, the mouse was placed in the same start arm as in Trial 1. The previously blocked arm was opened in Trial 2 and the mouse was allowed to investigate all three arms for 5 min (recall trial). The dependent variables measured in Trial 2 were: (1) the amount of time spent in each arm for each minute; (2) the number of entries made into each arm for each minute (Entry). Those indexs reflect inquisitive behavior (i.e. response to novelty) and spatial recognition memory of the previously unvisited arm. Step-down test: This test was used to measure inhibitory avoidance and short-term memory, according to the previously described method (PMID: 24678498). The apparatus comprised a plastic chamber (12x12x18cm) with an elevated rubber platform (4.84.84.5cm) placed on the left side wall. The floor was made of caliber stainless steel bars (0.1cm in length) placed in parallel, 0.5cm apart. On the first training day, mice were exposed to a 5-min learning course, if the animals stepped down from the platform, they were exposed to an electric foot shock (36V, AC). After 24h, latency was reassessed and recorded as the learning grade (latency), which was taken as a measure of memory retention. Each acquisition trial was performed 5min in the PKR+/+Tg+/- and PKR+/-Tg+/- mice. Supplementary Table 1. Primary antibodies used for protein immunodetection in western blot analysis (WB), immunohistochemistry (IHC) and immunofluorescence (IF). Antigen Supplier Application PAGE (%) Species origin Incubation conditions Ab dilution 8-OhdG US Biological, H9076-02 IF N/A Goat 10% NGS, 12h, 4à ¢Ã¢â‚¬Å¾Ã†â€™ 1:200 acetylated-ÃŽ ±-Tubulin Santa Cruz, sc- 23950 WB 10 Mouse 5% skim milk, 2h, O/N, 4à ¢Ã¢â‚¬Å¾Ã†â€™ 1:5000 APP Cell signaling, #2452 WB 10 Rabbit 5% skim milk, 2h, O/N, 4à ¢Ã¢â‚¬Å¾Ã†â€™ 1:1000 AT8 Thermo, MN1020B WB 10 Rabbit 5% skim milk, 2h, O/N, 4à ¢Ã¢â‚¬Å¾Ã†â€™ 1:1000 ATF-4 Abcam, ab50546 WB 10 Mouse 5% skim milk, 2h, O/N, 4à ¢Ã¢â‚¬Å¾Ã†â€™ 1:1000 ATF-4 Abcam, ab50546 IF N/A Mouse 10% NGS, 12h, 4à ¢Ã¢â‚¬Å¾Ã†â€™ 1:100 AÃŽ ²42 Abcam, ab10148 IHC N/A Rabbit 10% NGS, 12h, 4à ¢Ã¢â‚¬Å¾Ã†â€™ 1:100 BACE-1 Abcam, ab2077 WB 10 Rabbit 5% skim milk, 2h, O/N, 4à ¢Ã¢â‚¬Å¾Ã†â€™ 1:1000 CCS Abcam, ab16962 WB 10 Mouse 5% skim milk, 2h, O/N, 4à ¢Ã¢â‚¬Å¾Ã†â€™ 1:100 CHOP Cell signaling, #2895 WB 10 Mouse 5% skim milk, 2h, O/N, 4à ¢Ã¢â‚¬Å¾Ã†â€™ 1:1000 CREB Cell signaling, #9197 WB 10 Rabbit 5% skim milk, 2h, O/N, 4à ¢Ã¢â‚¬Å¾Ã†â€™ 1:1000 complexin-1/2 Santa Cruz, sc-33603 WB 10 Rabbit 5% skim milk, 2h, O/N, 4à ¢Ã¢â‚¬Å¾Ã†â€™ 1:1000 Cp Abcam, ab48614 WB 10 Rabbit 5% skim milk, 2h, O/N, 4à ¢Ã¢â‚¬Å¾Ã†â€™ 1:1000 Drebrin Cell signaling, #12243 WB 10 Rabbit 5% skim milk, 2h, O/N, 4à ¢Ã¢â‚¬Å¾Ã†â€™ 1:1000 eIF2ÃŽ ± Cell signaling, #5324 WB 10 Rabbit 5% skim milk, 2h, O/N, 4à ¢Ã¢â‚¬Å¾Ã†â€™ 1:1000 GSK-3ÃŽ ² Cell signaling, #9315 WB 10 Rabbit 5% skim milk, 2h, O/N, 4à ¢Ã¢â‚¬Å¾Ã†â€™ 1:1000 JNK Cell signaling, ##9252 WB 10 Rabbit 5% skim milk, 2h, O/N, 4à ¢Ã¢â‚¬Å¾Ã†â€™ 1:1000 Nitro-Tyrosine Cell signaling, #9691 WB 10 Rabbit 5% skim milk, 2h, O/N, 4à ¢Ã¢â‚¬Å¾Ã†â€™ 1:1000 NR2A Molecular Probes, A-6473 WB 8 Rabbit 5% skim milk, 2h, O/N, 4à ¢Ã¢â‚¬Å¾Ã†â€™ 1:500 NR2B Molecular Probes, A-6474 WB 8 Rabbit 5% skim milk, 2h, O/N, 4à ¢Ã¢â‚¬Å¾Ã†â€™ 1:500 PKR (N-Term) GenWay Biotech, GWB-A4757E WB 10 Rabbit 5% skim milk, 2h, O/N, 4à ¢Ã¢â‚¬Å¾Ã†â€™ 1:500 p-PKR (Thr 451) Invitrogen, 44668G WB 10 Rabbit 5% skim milk, 2h, O/N, 4à ¢Ã¢â‚¬Å¾Ã†â€™ 1:500 p-eIF2ÃŽ ± (Ser51) Cell signaling, #3398 WB 10 Rabbit 5% skim milk, 2h, O/N, 4à ¢Ã¢â‚¬Å¾Ã†â€™ 1:1000 p-GSK-3ÃŽ ² (Ser9) Cell signaling, #9336 WB 10 Rabbit 5% skim milk, 2h, O/N, 4à ¢Ã¢â‚¬Å¾Ã†â€™ 1:1000 p-CREB (Ser133) Cell signaling, #9198 WB 10 Rabbit 5% skim milk, 2h, O/N, 4à ¢Ã¢â‚¬Å¾Ã†â€™ 1:1000 p-JNK Cell signaling, #4671 WB 10 Rabbit 5% skim milk, 2h, O/N, 4à ¢Ã¢â‚¬Å¾Ã†â€™ 1:1000 p-PP2A Epitomics, 1155-1 WB 10 Rabbit 5% skim milk, 2h, O/N, 4à ¢Ã¢â‚¬Å¾Ã†â€™ 1:1000 PP2A C subunit Epitomics, 1512-1 WB 10 Rabbit 5% skim milk, 2h, O/N, 4à ¢Ã¢â‚¬Å¾Ã†â€™ 1:1000 PS396 Invitrogen, 44752G WB 10 Rabbit 5% skim milk, 2h, O/N, 4à ¢Ã¢â‚¬Å¾Ã†â€™ 1:1000 PS404 Invitrogen, 44-758G WB 10 Rabbit 5% skim milk, 2h, O/N, 4à ¢Ã¢â‚¬Å¾Ã†â€™ 1:1000 PSD-93 Cell signaling, #9445 WB 10 Rabbit 5% skim milk, 2h, O/N, 4à ¢Ã¢â‚¬Å¾Ã†â€™ 1:1000 PSD-95 Cell signaling, #2507 WB 10 Rabbit 5% skim milk, 2h, O/N, 4à ¢Ã¢â‚¬Å¾Ã†â€™ 1:1000 PSD-95 Cell signaling, #2507 IF N/A Rabbit 10% NGS, 12h, 4à ¢Ã¢â‚¬Å¾Ã†â€™ 1:100 synapsin 1 Invitrogen, 51-5200 WB 10 Rabbit 5% skim milk, 2h, O/N, 4à ¢Ã¢â‚¬Å¾Ã†â€™ 1:1000 sAPPÃŽ ± Covance, SIG-39139 WB 10 Rabbit 5% skim milk, 2h, O/N, 4à ¢Ã¢â‚¬Å¾Ã†â€™ 1:1000 sAPPÃŽ ² Covance, SIG-39138 WB 10 Rabbit 5% skim milk, 2h, O/N, 4à ¢Ã¢â‚¬Å¾Ã†â€™ 1:1000 Tau-1 Chemicon, MAB3420 WB 10 Mouse 5% skim milk, 2h, O/N, 4à ¢Ã¢â‚¬Å¾Ã†â€™ 1:1000 Tau-5 Abcam, ab80579 WB 10 Mouse 5% skim milk, 2h, O/N, 4à ¢Ã¢â‚¬Å¾Ã†â€™ 1:500 ÃŽ ±-tubulin Santa Cruz, sc-58667 WB 8-10 Mouse 5% skim milk, 2h, O/N, RT 1:1000 ÃŽ ²-actin Santa Cruz, sc-47778 WB 10 Mouse 5% skim milk, 2h, O/N, RT 1:1000 N/A, not applicable; NGS, normal-goat serum; O/N, over-night; RT, room temperature. SUPPLEMENTARY FIGURES Supplementary Figure 1. Content of Cu in Serum and brain. (A-D) Total iron, zinc, calcium, magnesium content in the serum respectively; (E-H) Total iron, zinc, calcium, magnesium content in the hippocampus respectively; (I-L) Total iron, zinc, calcium, magnesium content in the cortex respectively; *P

Contrasting Yeats’ Second Coming and Shelleys Ozymandias Essay

Contrasting Yeats’ Second Coming and Shelley's Ozymandias      Ã‚   William Butler Yeats specialized in the early Modernists style of literature.   Coming just out of the Late Victorian age, Yeats used strong literary and historic elements in literary form to evoke his symbolic message in "The Second Coming."   Through the use of his theme of the "new Apocalypse," (lecture notes on Early 20th Century Modernism) he imagined the world was coming into a state of unsurity from the post-WWI Modernist experience.   The war left people in a state of chaos, and although the war was meant to bring people a sense of hope for no more wars in the future, it did far more damage then good, especially in people's minds.   The time in the Modernist era was reflected in the equally chaotic, and choppy word structure in Yeats' poem.   In "The Second Coming" conditions are illustrated as being chaotic, "Things fall apart; the centre cannot hold;/ Mere anarchy is loosed upon the world" (Yeats, Longman p. 2329: ll. 3-4), confused in a way.   Those wo rds he uses, "fall apart," "cannot hold," and "anarchy" are ...

Conversation Essay

Date Conversations We use interpersonal communication every day in our live. There are three types of communication model, linear, interactive, and transactional communication. Each type of communication model has their strength and weakness. Linear communication is simple and straightforward but it does not totally describe what you wanted to say because it is a one way communication and short. Interactive communication can captures a variety of communication forms but less active and not much of collaborative in both party to interpret meanings because it neglects the active role in senders and receivers.Transactional communication gives you time to collaborates and to captures what most people thinks, mostly face to face communication, and this model enable both party to constantly exchange nonverbal and verbal messages. Interpersonal communication is an important tool to learns and to builds up your communication skill because they are essential to an interpersonal relationship. It is difficult to build a relationship with someone if interpersonal communication skill is poor. Interpersonal communication helps us recognize concepts and awareness that influences our thoughts, emotion and behaviors in a relationship.I remembered of my first date with Talia, our conversation it was not as smooth or friendly. We were partner up by our instructor, not by our free will, and this created a barrier between at first; especially that we are both old types person that came from different culture, ethnic and have different ethics. They say first impression is very important when you approach someone because interpersonal communication is irreversible, so I was very cautious or self-aware when I interacted with Talia. Mostly we started out the conversation with a question, and then each of us takes turns to response to the question.Half way through the conversation, I noticed that we used fewer questions to start out a new conversation, and we go to straight to the subje cts. Our different ethnic’s background intertwined our ethics which influenced our behaviors during the conversation, but we had good eyes contact and body gestures. Knowing my partner’s background, and culture made the conversation more interesting and dynamic. We had our third conversation date was failed because we set the time up already but I was unable to pick up the phone at work.During the time Talia gave me a call suddenly the customer came to eat and I was busy at that time. Normally, at the time we set up my uncle restaurant was not busy but I do not why this time so busy. I missed the conversation appointment with Talia. I know this is my false. After I received her massage I tried to give her a call back but she did not pick up the phone either. And then I try text her message but the result I got from her was busy too. I know we all busy because we have many thing to take care.But any way we were failed on third conversation, I know that each person has t o responsibility with the first thing we have to deal with. Interpersonal communication skill is essential to interpersonal relationship. It helps us understand and recognizes concepts to build a better relationship, whether it’s for business, friendship, or romantic relationship. Understand interpersonal communication helps you prevent or dealing with dysfunctional relationship, and builds better social networks around you. I believed this skill also helps build your self-esteem, openness, maintaining relationship and a well-being life.

Civil War, the ulmination of four decades of intense sectional conflict of social and political difference between the North and the South

Civil War, the ulmination of four decades of intense sectional conflict of social and political difference between the North and the South The Civil War was the culmination of four decades of intense sectional conflictof social and political difference between the North and the South. Slavery played a big part in the cause of the civil war, north not believing in slavery and the south slavery was praised. Slaves was the largest single investment in the south, the labor in the south including nearly four million enslaved blacks. 'The Civil War stands not only as a steppingstone to the lighting war - blitzkrieg- of 1939 to 1945. Politically a civil war, militarily it was actually a conflict between two hostile nations- two peoples divided by an unbeatable difference of opinion on the right of secession.'The question whether slavery should be permitted enter the territories was an big issue in American politics. The slavery issue was first arisen in 1819 when Missouri applied for admission as a state that allowed slavery, there would be a problem.kansasThe slavery issue was never resolved for years, but criticism of slaver y hung around for years. The northern began to say that slavery should be abolished or faded out during a period of time.As the issue deepened , three proposals to solve the situation were advanced. Most southern contended that slaveholders had a constitutional right to take their property into recently acquired territory. The most confirmed antislavery northern held that congress had a constitutional power and a moral duty to exclude the institution from the new territory or from any other territory. They believed that if slavery was prevented from expanding , it would die by nature.Another answer to the question on the new territory was POPULAR SOVEREIGNTY , which meant that slaveholders should be allowed to decide whether or not if they want slavery in the state. The issue of slavery should...

Racism and Segregation

Racism and Segregation Introduction In the book, I Know Why the Caged Bird Sings, the author, Maya Angelou, describes how she grew up as an intelligent Afro-American girl in South America and later in California in 1940’s. She gives an account of the various characteristics of her society that made her to mature. Having worked with Martin Luther in combating racism before her book, she had to incorporate the theme of racism in her work. Thus racism and segregation is highly depicted as a theme in her book (Angelou 1).Advertising We will write a custom essay sample on Racism and Segregation specifically for you for only $16.05 $11/page Learn More Racism and Segregation In this book, Maya comes across many instances of blatant racism. She is forced to face racism and segregation in her country at a very young age. While living in Stamps, Arkansas, she experiences the region’s characteristic segregation to the extent that she believes that white people do not exist. At this tender age, she is made to believe that every good thing belongs to the whites. This is evidenced in many incidences. An example is that, as she fails to recite her poem in church, she notes that her dress is probably a handout from a white woman. She associates blond hair with beauty and believes that she is trapped in a â€Å"black ugly dream† (Angelou 7) with an unattractive African-American body. She also believes that she will soon wake from her nightmare and become as beautiful as the whites. The segregation that is characteristic of Stamps is evidenced by the statement that Mrs. Henderson owns a store in the black segment of Stamps (Angelou 1 11). As she grows older, Maya encounters racist incidences that are more open and personal. Some of these incidences include the demeaning address that is directed towards her by a white speaker during her eighth-grade graduation, she also faces racist problems while applying for a streetcar job but she overcomes the problem s to get the job. Her white boss is also depicted as a racist. He calls her Mary despite her disapproval. When she visits a white dentist, he declines to treat her. There is evidence that black people were prone to acts of violence perpetrated to them by their white counterparts. This is from the incidence in which Maya and Willie help a black man escaping a white lynch mob. The importance that the black attach to the world championship boxing match of Joe Louis shows how the black people thirst for recognition and also proves inequality in the community. Maya is also portrayed as a racist in some ways. For instance, she feels guilty of loving the works of William Shakespeare due to the mere fact that he was white (Angelou 11 23). In summary, the book has a notable thematic bias on racism.Advertising Looking for essay on literature languages? Let's see if we can help you! Get your first paper with 15% OFF Learn More Conclusion From the discussion above, it is clear that the community Maya is living in is faced with serious segregation and racism. Maya uses this community to describe how life was during her time. She creates events that depict how racism was deeply rooted in the American community in the 1940’s. As described, these events include her belief that black people are ugly, the derogatory address by a white speaker during her graduation, the refusal by a white dentist to treat her, etc (Angelou 10 24). Through these experiences, she learns how racism and segregation has affected her family members’ characters and strives to overcome the pressure mounted on her by these social problems. Angelou, Maya. I Know Why the Caged Bird Sings. U.S. Bantam Books, 1983. Print.

Experimental Design for statistics Essay Example | Topics and Well Written Essays - 500 words

Experimental Design for statistics - Essay Example They will be required to be in the first month of the pregnancy so that they can be monitored from early stages in their pregnancy development. Fresh mothers will not be required to take part in this study but only mothers who have at least one other child. A sample of about 200 mothers will be selected for this study. The sample will be representative of all the population. Mothers will have their blood pressure measured to ascertain that they are healthy to take place in this study. The child’s brain development will be measured using the Magnetic Resonance Imaging (MRI) which measures the brain and behaviour development. This will be done as the child grows, probably up to 12 years. Further, the kids will have their IQ tested through simple tasks, their social behaviors and their computation skills. All this measurements will be recorded and put into a computer package for analysis. Based on the kind of fish his/her mother was consuming, the level of the IQ, their social behaviours and computing skills will be compared. The level of the MRI will say whether that certain kind of fish will have detrimental effects. Pregnant mothers are classified as vulnerable people thus; their protection from harm will be a priority. Further, their privacy will be protected to the highest level. Also, they will be taken through all the information concerning this study so as to make an informed decision about taking place in the study. No coercion will be used at

Computer-Based information system Essay Example | Topics and Well Written Essays - 500 words

Computer-Based information system - Essay Example The paper will also discuss the computer-based information system of AT&T were misused by employees. The cloud-based data store is one of the recent information systems that was developed by AT&T Telecommunication Company. An article that was published by computer world indicated that the system is designed to help enterprise-based developers in the usage of (IoT) Internet of Things apps, as well as services. The system is designed to allow collection and the analysis of data more comfortable from various remote devices that are interconnected to large businesses (Hamblen, 2015). AT&T Company has also developed Monitoring systems for organizations. Currently, organization performance has been the primary focus for most organizations. Managers have been struggling on how they can improve the performance of their organizations by increasing performance by increasing supervision with a slight budget line. Monitoring systems have played a significant role in improving performance of various organizations (Computerworld, 1983). Computer based information system of AT&T has significantly reduced the cost of operations to most organizations. For instance, the development of cloud data stores played a significant role in cutting down the cost of hiring employees for a considerable number of organizations (AT&T Tech, 2012). Initially, most computer-based information systems of AT&T were predestined to improve the performance of the organization by either cutting down the cost of resources or improving the quality of services. Conversely, most information based system has been misused by employees for their personal gain. For instance, monitoring systems are designed to either monitor the performance of employees by the supervisors. A great part of monitoring systems is a computerized collection, analysis, storage as well as reporting